Thank you for the introduction.

First of all, Johannes and Alexander, thank you for organizing very nice locations.

I'm very happy to be here to talk about my recent researches.

Before going into this, this is a picture of 2013 when we had AUC 2013 in Japan.

You can find the helmet there.

And so several also the AUC folks, but AUC giants like Tom Lawe and also Walter Stafford were there.

And that was very nice meeting in Japan.

And so also I'm also a big fan of AUC.

So I started, so maybe I didn't know dark age.

I started AUC as dark age, but my initial AUC was model E and manufactured in 1970, but almost I think a final version of model E.

So this was highly, so many parts I equipped, including high temperature unit.

So this is up to 80 degree.

So the model E was actually, we can measure even 80 degree for mostly I think polymer science purposes.

And what I did is so that every part, including optics, UV scanner was removed and instead designed for like laser CCD camera.

And also everything was digitalized because I wanted to analyze by my own PC.

But soon after XLA was delivered.

And so when I first used the actual program, so AUC very specialized program is DCDT by Walter Stafford.

So he sent me personally.

So this DCDT and that was now Mac, very Apple computer initially.

And then next XLI was installed and I started to use Ultrascan and to learn to going to Boris's place in San Antonio.

And also I started to use SEDFIT.

But these instruments all retired at this moment.

And active instrument in my laboratory is XLI and XLF fluorescent detector modified XLA.

This is Aviv type fluorescent detector.

And this optimize mostly used academics purpose.

And also this is quite kind of a business purpose like a GMP AUC.

And also one more AUC will coming soon.

Optima AUC will coming soon.

So at this moment this is current, I mean the circumstance for the AUC.

So I really like to use AUC.

But my project is this.

So what I'm doing is responsibility of the manufacturing and the quality control of the virus vector for gene therapies in mostly for academic hospitals.

So some pipeline will come for academics or company.

And so we make, we do process development starting from the culture, condition and downstream process.

And so our actually manufactured vector can be used for animal talk studies.

And so usually 50 or 80 AAV is manufactured in our laboratory every year.

And all important point is all manufactured AAV is analyzed by density gradient ultracentrifugation.

That is automatically online detector having online detector and also band sedimentation AUC.

So we are a lot of analysis of the AUC for AAV viruses and also lentiviruses recently.

And also some company request us for the analytics only for so that we are providing non-GMP and GMP AUC for.

This is a kind of CDMO function in university.

And not only AUC, this is actual picture in my laboratory for starting a culture like this.

So this is human cell culture.

And typically one liter culture cost 5000 to us.

So very, very expensive experiment this one.

So the extensive fabrication like chromatography is used.

And also about analysis, digital PCR capillary electrolysis mass photometry.

And also I have cryo-EM on my laboratory and for just full and empty classification.

And also some DLS and also five or six mass spec for characterizing AAV.

So this is current situation in my laboratory.